Journal: Cell and Tissue Research

Article Title: Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury

doi: 10.1007/s00441-020-03173-1

Figure Lengend Snippet: Antibodies

Article Snippet: Primary , Luc2 , Mouse , monoclonal , DSHB , DSHB-LUC-2 , Cytoplasm , 25:100 , Luc2-copGFP transduced cells.

Techniques: Control

Journal: Cell and Tissue Research

Article Title: Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury

doi: 10.1007/s00441-020-03173-1

Figure Lengend Snippet: In vitro differentiation and loading with ferumoxytol of transduced HFBSCs. Prior to differentiation (day − 1) pCDH-EF1α-Luc2-T2A-copGFP-transduced cells exhibited a bright green-fluorescent signal of copGFP (a) and had normal morphologies (a″; phase contrast). The merged image (a‴) demonstrates that all cells were transduced with the reporter gene construct. Within 7 days, HFBSCs adapted neuronal morphologies (b; copGFP fluorescence and b′; Phase contrast). Scale bar is 100 μm. HFBSCs transduced with the pCDH-DCX-Luc2-T2A-copGFP construct did not express copGFP (or Luc2) prior to differentiation (day − 1) as indicated by the absence of a fluorescent signal (c). However, cells expressed copGFP under regulation of the DCX promoter as indicated by the green fluorescent signal 7 days after start of the differentiation (d). HFBSCs also adapted neuronal morphologies (d′; phase contrast). Scale bar is 100 μm. Perls’ Prussian blue (PB) staining + DAB intensification stained ferumoxytol within the cells as marked by a brown precipitate (e′). Faint copGFP fluorescence persisted through the staining process as can be observed in the fluorescence (e) and merged images (e″). Scale bar is 100 μm

Article Snippet: Primary , Luc2 , Mouse , monoclonal , DSHB , DSHB-LUC-2 , Cytoplasm , 25:100 , Luc2-copGFP transduced cells.

Techniques: In Vitro, Transduction, Construct, Fluorescence, Staining

Journal: Cell and Tissue Research

Article Title: Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury

doi: 10.1007/s00441-020-03173-1

Figure Lengend Snippet: Observation of Luc2 activity in TBI mice in vivo. Representative overlays of pCDH-EF1α-Luc2-T2A-copGFP-transduced HFBSCs from 2 (a), 14 (a′), 33 (a″) and 49 days after transplantation (a‴). The bioluminescent signal increased with relative stability over the course of time. Representative overlays of HFBSCs transduced with pCDH-DCX-Luc2-T2A-copGFP over the same period of time. No bioluminescent signal was observed 2 days after transplantation (b). The signal increased between 14 days (b′) and 33 days (b″) but was almost undetectable after 49 days (b‴). Analysis of the bioluminescent signal was measured 2, 14, 33 and 49 days after transplantation (c). The bioluminescence data were normalized with the initial signal and measured 2 days after transplantation, which depicts the trend of the bioluminescent signal from HFBSCs over time. The bioluminescent signal of pCDH-EF1α-Luc2-T2A-copGFP-transduced HFBSCs increased steadily over the course of time, while the bioluminescence of pCDH-DCX-Luc2-T2A-copGFP-transduced HFBSCs decreased after 33 days and was almost undetectable at 49 days

Article Snippet: Primary , Luc2 , Mouse , monoclonal , DSHB , DSHB-LUC-2 , Cytoplasm , 25:100 , Luc2-copGFP transduced cells.

Techniques: Activity Assay, In Vivo, Transplantation Assay, Transduction

Journal: Cell and Tissue Research

Article Title: Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury

doi: 10.1007/s00441-020-03173-1

Figure Lengend Snippet: Immunohistochemical staining of HFBSCs constitutively expressing Luc2 and copGFP. Sections of mouse brains containing transduced HFBSCs exhibited native green fluorescence emitted by copGFP after fixation, sectioning and staining of the sections (a, b, c, d; green). Sections containing copGFP-expressing HFBSCs were stained for either copGFP (a′ and c′) or Luc2 (b′ and d′). CopGFP-expressing HFBSCs stained for copGFP (a′; red) and the neural progenitor cell marker nestin (a″; gray). The merged image (a′′′′) shows colocalization of copGFP (a′), nestin (a″) and DNA (a‴) of copGFP-expressing HFBSCs in the mouse brain. HFBSCs, which expressed copGFP (b; green), also stained for Luc2 (b′; red). GFAP (b″; gray) stained in the mouse brain but is absent in HFBSCs (b‴; green/red). Transplanted HFBSCs (c/c′; green/red) stained for NF-Pan (c″; gray). None of the copGFP-expressing HFBSCs (d/d′; green/red) stained for DCX (d″). Scale bar = 50 μm

Article Snippet: Primary , Luc2 , Mouse , monoclonal , DSHB , DSHB-LUC-2 , Cytoplasm , 25:100 , Luc2-copGFP transduced cells.

Techniques: Immunohistochemical staining, Staining, Expressing, Fluorescence, Marker

Journal: Cell and Tissue Research

Article Title: Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury

doi: 10.1007/s00441-020-03173-1

Figure Lengend Snippet: Immunohistochemical staining for extracellular matrix and proliferating cells. CopGFP-expressing HFBSCs in mouse brain sections exhibited native fluorescence (a, b, and c; copGFP protein; green). Additionally, sections were stained for Luc2 (a′, b′, and c′; red). The surrounding area of some HFBSCs stained for fibronectin (a″; gray). The vicinity of transplanted HFBSCs stained for laminin (b″; gray). Staining for Ki-67 (c″; gray) was negative in sections containing HFBSCs (green/red). Scale bar = 50 μm

Article Snippet: Primary , Luc2 , Mouse , monoclonal , DSHB , DSHB-LUC-2 , Cytoplasm , 25:100 , Luc2-copGFP transduced cells.

Techniques: Immunohistochemical staining, Staining, Expressing, Fluorescence

Journal: Cell and Tissue Research

Article Title: Multimodal imaging of hair follicle bulge-derived stem cells in a mouse model of traumatic brain injury

doi: 10.1007/s00441-020-03173-1

Figure Lengend Snippet: Overview of staining pattern

Article Snippet: Primary , Luc2 , Mouse , monoclonal , DSHB , DSHB-LUC-2 , Cytoplasm , 25:100 , Luc2-copGFP transduced cells.

Techniques: Staining, Cell Culture, Expressing, Migration, Cell Attachment Assay

Journal: British Journal of Pharmacology

Article Title: The nociceptin/orphanin FQ receptor system as a target to alleviate cancer‐induced bone pain in rats: Model validation and pharmacological evaluation

doi: 10.1111/bph.14899

Figure Lengend Snippet: Levels of inflammatory markers measured in plasma of sham and 1.5 × 10 6 ·ml −1 of MRMT‐1/Luc2‐bearing animals

Article Snippet: The rat mammary gland carcinoma cell line, MRMT‐1 (Tohoku University, JP; TKG Cat# TKG 0132, RRID:CVCL_5156), was transfected with the luciferase (Luc2) gene (Trenzyme, Life Science Services, DE) to allow monitoring of cell presence and tumour growth over time.

Techniques: Clinical Proteomics, Marker

Journal: British Journal of Pharmacology

Article Title: The nociceptin/orphanin FQ receptor system as a target to alleviate cancer‐induced bone pain in rats: Model validation and pharmacological evaluation

doi: 10.1111/bph.14899

Figure Lengend Snippet: Gene expression of oprl1 in dorsal root ganglion (DRG) and bone marrow, measured 21 days post‐surgery in sham and 1.5 × 10 6 ·ml −1 of MRMT‐1/Luc2‐bearing animals

Article Snippet: The rat mammary gland carcinoma cell line, MRMT‐1 (Tohoku University, JP; TKG Cat# TKG 0132, RRID:CVCL_5156), was transfected with the luciferase (Luc2) gene (Trenzyme, Life Science Services, DE) to allow monitoring of cell presence and tumour growth over time.

Techniques: Gene Expression

Journal: British Journal of Pharmacology

Article Title: The nociceptin/orphanin FQ receptor system as a target to alleviate cancer‐induced bone pain in rats: Model validation and pharmacological evaluation

doi: 10.1111/bph.14899

Figure Lengend Snippet: Effect of 1.25 × 10 6 ·ml −1 of MRMT‐1/Luc2 inoculated cells on (a) weight‐bearing ratio, (b) cold allodynia, and (c) mechanical allodynia, including the effect of i.p. morphine (3.16 mg·kg −1 ) administration. All data are presented as mean ± SEM; n = 9, all MRMT‐1/Luc2‐bearing animals; * P < .05 versus pretest

Article Snippet: The rat mammary gland carcinoma cell line, MRMT‐1 (Tohoku University, JP; TKG Cat# TKG 0132, RRID:CVCL_5156), was transfected with the luciferase (Luc2) gene (Trenzyme, Life Science Services, DE) to allow monitoring of cell presence and tumour growth over time.

Techniques:

Journal: British Journal of Pharmacology

Article Title: The nociceptin/orphanin FQ receptor system as a target to alleviate cancer‐induced bone pain in rats: Model validation and pharmacological evaluation

doi: 10.1111/bph.14899

Figure Lengend Snippet: (a) The effect of nociceptin (i.t.) and morphine (i.t.) and (b) the effect of systemic Ro65‐6570 (i.p.; NOP receptor agonist) and morphine (i.p.) on mechanical allodynia in 1.5 × 10 6 ·ml −1 of MRMT‐1/Luc2‐bearing animals in the von Frey test. (c) The effect of Ro65‐6570 (i.p.) is blocked by the NOP receptor antagonist J‐113397 (i.p.). All data are presented as mean ± SEM; n = 7 sham, n = 8 vehicle, n = 7 morphine, n = 8 nociceptin 1 μg, n = 8 nociceptin 3 μg, n = 6 nociceptin 10 μg in (a); n = 9 sham, n = 8 vehicle, n = 6 morphine, n = 7 Ro65‐6570 0.3 mg·kg −1 , n = 6 Ro65‐6570 1.0 mg·kg −1 , n = 5 Ro65‐6570 2.15 mg·kg −1 in (b); n = 9 vehicle, n = 8 Ro65‐6570, n = 8 J‐113397, n = 10 Ro65‐6570 + J‐113397 in (c); * P < .05 versus vehicle

Article Snippet: The rat mammary gland carcinoma cell line, MRMT‐1 (Tohoku University, JP; TKG Cat# TKG 0132, RRID:CVCL_5156), was transfected with the luciferase (Luc2) gene (Trenzyme, Life Science Services, DE) to allow monitoring of cell presence and tumour growth over time.

Techniques: